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西宁及其周边地区近年来出现一种疑似白菜黑胫病的新病害。为明确其病原，对西宁地区采集的3份疑似白菜黑胫病病样开展了研究，从病原菌组织分离获得10株分离物，对2株代表性菌株QK6和QK10进行了形态学观察、DNA序列分析及致病性测定。形态学显示，2株代表性菌株菌落形态有所不同，但分生孢子器均为表生，单生，呈球形或近球形，黑色；产孢细胞光滑，无色透明，为安培形；分生孢子无色透明，无隔，呈柱状或卵圆形。基于ITS序列的系统发育分析表明，QK6和QK10与Plenodomus biglobosus聚在同一分支。致病性测定证实2株菌株均可侵染白菜引起黑胫症状。综上，西宁地区疑似白菜黑胫病病原鉴定为P. biglobosus，这是该地区首次报道的一种真菌新病害。
Abstract：A new disease suspected to be blackleg disease of Chinese cabbage has emerged in Xining city in recent years. In order to identify the pathogen,three samples of blackleg disease of Chinese cabbage were collected in Xining city. Ten strains were isolated and obtained,and two representative strains QK6 and QK10 were subjected to morphological observation,DNA sequence analysis and pathogenicity test. Morphological observation showed the two representative strains exhibit distinct colony morphologies.However,both strain's pycnidia were supergene,solitary,globose or subglobose and black. The sporogenous cells were smooth,colorless and transparent,ampere shape. Conidia were colorless,transparent,septate,columnar or ovoid. Phylogenetic analysis showed that QK6 and QK10 clustered in the same branch with Plenodomus biglobosus. Pathogenicity test confirmed that the two strains could infect Chinese cabbage and induce blackleg symptoms. Based on these results,the pathogen was identified as P. biglobosus,which was a new fungal disease reported for the first time in Xining city.
[1] WANG J J. Production status and development countermeasures of Chinese cabbage in China(in Chinese). China Fruit&Vegetable,2020,40(7):80-82.
[2]张鹤，郭竞，路翠玲，等.早熟大白菜品种（系）比较实验.中国瓜菜，2018,31(1):35-37.
[3]刘双如，孔小平，张晓梅，等.青海白菜黄萎病病原菌鉴定.植物病理学报，2024,54(6):1286-1290.
[4]王一丹，魏立娟，杨成德.甘肃省大白菜叶斑病菌分离鉴定及生物学特性测定.西北农业学报，2023,32(10):1637-1645.
[5]方中达.植病研究方法.第3版.北京：中国农业出版社，1998:122-126.
[6] WHITE T J,BRUNS T,LEE S,et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics//INNIS M A,GELFAND D H,SNINSKY J J,et al.PCR Protocols:a guide to methods and applications. San Diego,California:Academic Press,1990:315-3226.
[7] GABRIELSON R L. Blackleg disease of crucifers caused by Leptosphaeria maculans(Phoma lingam)and itscontrol. Seed Sci and Technol,1983,11(3):749-780.
[8] CAI X,YANG L,ZHANG J,et al. First report of Leptosphaeria biglobosa causing black leg on Brassica campestris ssp. chinensis var. purpurea in central China. Plant Dis,2014,98(8):1156.
[9] LUO T,CAI X,LI G,et al. First report of Leptosphaeria biglobosa causing blackleg on Broccoli(Brassica oleracea var.italica)in central China. Plant Dis,2018,102(6):1178.
[10] ZHOU K,YU C,CAI X,WU M D,et al. First report of Leptosphaeria biglobosa‘brassicae’causing black-leg on Brassica juncea var. multiceps in China. Plant Dis,2019,103(1):160.
[11] DENG Y,YU C,WU M D,et al. First report of Leptosphaeria biglobosa'brassicae'causing blackleg on Brassica juncea var. tumida in China. Plant Dis,2020,104(3):984.
[12] LONG Y,SHANG M,DENG Y,et al. First report of Leptosphaeria biglobosa'brassicae'causing blackleg on Brassica juncea var. multisecta in China. Plant Dis,2021,105(11):3749.
[13] YU C,DENG Y,WU M D,et al. First report of Leptosphaeria biglobosa'brassicae'causing blackleg on Brassica rapa subsp.pekinensis in China. Plant Dis,2021,105(10):3305.
[14] ROUXEL T,BALESDENT M H. The stem canker(blackleg)fungus,Leptosphaeria maculans,enters the genomic era. Mol Plant Pathol,2005,6(3):225-241.
[15] FITT B D L,BRUN H,BARBETTI M J. et al. World wide importance of phoma stem canker(Leptosphaeria maculans and L. biglobosa)on oilseed rape(Brassica napus). European Journal of Plant Pathology,2006,114:3-15.
[16]李经略，柯桂兰，赵稚难.大白菜黑胫病调查鉴定简报.陕西农业科学，1989(6):28.
[17] CAI X,YANG L,ZHANG J,et al. First report of Leptosphaeria biglobosa causing blackleg on Raphanus sativus in central China. Plant Disease,2014,98(7):993.
[18] LUO T,CAI X,LI G,et al. First report of Leptosphaeria biglobosa causing blackleg on broccoli(Brassica oleracea var.italica)in central China. Plant Disease,2018,102(6):1178.
[19]李欣洲，宋培玲，郝丽芬，等.油菜黑胫病菌（Leptosphaeria biglobosa）侵染条件研究.华北农学报，2013,28(2):235-238.
[20] CAI X,HUANG Y,JIANG D,et al. Evaluation of oilseed rapeseed yield losses caused by Leptosphaeria biglobosa in central China. European journal of plant pathology,2018,150(1):179-190.
[21]荣松柏，胡宝成，陈凤祥，等.油菜黑胫病对油菜产量及农艺性状的影响.作物杂志，2015(6):159-161,167.
[22] MAHUKU G S,GOODWIN P H,HALL R. A competitive polymerase chain reaction to quantify DNA of Leptosphaeria maculans during blackleg development in oilseed rape. Molecular Plant-Microbe Interactions,1995,8(5):761-767.
[23] MAHUKU G S,HALL R,GOODWIN P H. Distribution of Leptosphaeria maculans in two fields in southern Ontario as determined by the polymerase chain reaction. European Journal of Plant Pathology,1996,102(6):569-576.
[24] LIU S Y,LIU Z Y,FITT B D L,et al. Resistance to Leptosphaeria maculans(phoma stem canker)in Brassica napus(oilseed rape)induced by L. biglobosa and chemical defence activators in field and controlled environments. Plant Pathology,2006,55(3):401-412.
[25]周国梁，尚琳琳，于翠，等.进境油菜籽中黑胫病菌和茎基溃疡病菌的检测.植物保护学报，2010,37(4):289-294.
[26]易建平，周国梁，印丽萍，等.进境澳大利亚油菜籽中茎基溃疡病菌的检测.植物病理学报，2010,40(6):70-73.
[27]陈青，黄峰，廖富荣，等.进境青菜种子携带油菜茎基溃疡病菌的检测与鉴定.植物检疫，2013,27(4):62-64.
[28] FERNANDO W,ZHANG X H,CHAMI A. Detection of Leptosphaeria maculans and Leptosphaeria biglobosa causing blackleg disease in canola from canadian canola seed lots and dockage. Plants,2016,5(1):12.
[29]周国梁，尚琳琳，林泓，等.油菜茎基溃疡病菌的实时荧光PCR检测.植物病理学报，2011(1):12-19.
[30] SONG P,J?E DRYCZKA M,IRZYKOWSKI W,et al. Efficient detection of Leptosphaeria maculans,from infected seed lots of oilseed rape. Journal of Phytopathology,2016,164(11/12):1097-1104.
[31]周圆，黄海龙，李孝军，等.油菜茎基溃疡病菌LAMP-LFD检测方法的建立.植物检疫，2016,30(4):32-37.
[32] MENDES-PEREIRA E,MARIE-H魪LèNE B,BRUN H,et al.Molecular phylogeny of the Leptosphaeria maculans-L. biglobosa species complex. Mycological Research,2003,107(11):1287-1304.
[33] VOIGT K,COZIJNSEN A J,KROYMANN J,et al. Phylogenetic relationships between members of the crucifer pathogenic Leptosphaeria maculans species complex as shown by mating type(MAT1-2),actin,and betatubulin sequences. Molecular Phylogenetics&Evolution,2005,37(2):541-557.
[34] VINCENOT L,BALESDENT M H,LI H,et al. Occurrence of a new subclade of Leptosphaeria biglobosa in Western Australia. Phytopathology,2008,98(3):321-329.
[35] https：//www.indexfungorum.org/.
[36] DE GRUYTER J,WOUDENBERG J H C,AVESKAMP M,et al. Redisposition of Phoma-like anamorphs in Pleosporales.Studies in Mycology,2013,75:1-36.
[37] ZHAO P,CROUS P W,HOU L W,et al. Fungi of quarantine concern for China I:Dothideomycetes. Persoonia,2021,47:45-105.
基本信息:
DOI：10.19662/j.cnki.issn1005-2755.2025.03.003
中图分类号:S436.34
引用信息:
[1]张晓梅,刘双如,刘芳等.西宁市白菜黑胫病病原鉴定[J].植物检疫,2025,39(03):15-21.DOI:10.19662/j.cnki.issn1005-2755.2025.03.003.
基金信息:
青海省基础研究计划项目（2022-ZJ-726）; 西宁市重大科技创新平台能力建设专项（2022-Z-02）