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烟草环斑病毒（tobacco ringspot virus,TRSV）分布于50多个国家，是我国进境植物检疫性病毒。该病毒的寄主范围广，可侵染54科300多种植物；通过机械方式、嫁接、线虫媒介以及种子传播，对作物产量和品质时常造成严重损失。本研究选择了TRSV的外壳蛋白基因序列设计引物，建立了基于反转录（reverse transcription,RT）-重组酶聚合酶扩增（recombinase polymerase amplification,RPA）和侧流层析试纸条（lateral flow dipstick,LFD）的RT-RPA-LFD检测方法，灵敏度为486 pg/μL。验证结果表明本研究所设计的RPA引物特异性强，操作简便，适用于田间或口岸现场，利于TRSV的监测与暴发流行的早期预警。
Abstract：Tobacco ringspot virus(TRSV) is prevalent in over 50 countries worldwide,and has been categorized as a plant quarantine pest in China. TRSV has a wide host range,infecting over 300 plant species in 54 families. TRSV spreads through mechanical means,grafting,nematodes and seeds,posing a significant risk of epidemics that could severely impact crop yield and quality. A detection system combining reverse transcription(RT), recombinase polymerase amplification(RPA) and lateral flow dipstick(LFD),has been established using primers based on the coat protein gene sequence. This method exhibited a sensitivity of 486 pg/μL,and specificity validation demonstrated that the RPA primers designed in this study are highly specific. The method is simple to perform,thus suitable for on-site or field detection of TRSV.This approach could be of significant importance for the monitoring and early prediction of TRSV occurrence and outbreaks.
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基本信息:
DOI：10.19662/j.cnki.issn1005-2755.2025.01.003
中图分类号:S41-30
引用信息:
[1]韩春雪,徐司琦,王欣宇等.基于RT-RPA和试纸条的烟草环斑病毒可视化快速检测技术研究[J].植物检疫,2025,39(01):12-18.DOI:10.19662/j.cnki.issn1005-2755.2025.01.003.
基金信息:
国家重点研发计划项目（2022YFC2601500;2022YFC2601503）; 三亚崖州湾科技城管理局资助项目（SYND-2021-03）