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在大连开展松林中松材线虫调查时，从濒死马尾松中分离到一种滑刃属线虫群体，均为雌虫。该种线虫形态学主要鉴定特征为虫体细长，侧线4条，排泄孔位于神经环前或神经环对应位置，尾呈圆锥形，略腹弯，尾端有2～4个小突起，呈星状尾尖突。该大连群体28S rRNA D2/D3序列与GenBank中假古德伊滑刃线虫序列相似度达99%以上，系统发育树显示大连线虫种群与假古德伊滑刃线虫聚在一个分支上。经形态特征比较和28S rRNA D2/D3基因序列分析，将该线虫鉴定为假古德伊滑刃线虫。这是我国关于该种线虫存活于松木中的首次报道。
Abstract：During the investigation of pine wood nematodes in Dalian,a population of nematodes belonging to the genus Aphelenchoides was isolated from dying Pinus massoniana. Only females were found.The main morphological identification characteristics of this species of nematode are that the slender body,4 lateral lines,the excretory pore is located in front of or opposite the nerve ring,the tail is conical,slightly curved ventrally,and there are 2 to 4 small protrusions at the tail end,presenting a star-shaped caudal tip process. The similarity between the 28S rRNA D2/D3 sequences of the Dalian population and the Aphelenchoides pseudogoodyi sequences in GenBank is over 99%. The phylogenetic tree shows that the Dalian nematode population clusters into one clade with Aphelenchoides pseudogoodyi. Through the comparison of morphological characteristics and the analysis of the 28S rRNA D2/D3 gene sequence,it has been identified as Aphelenchoides pseudogoodeyi. This is the first report in China of this species of nematode surviving in pine wood.
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基本信息:
DOI：10.19662/j.cnki.issn1005-2755.2025.03.005
中图分类号:S763
引用信息:
[1]马欣欣,赵亚津,顾建锋.大连松木中假古德伊滑刃线虫形态和分子鉴定[J].植物检疫,2025,39(03):29-34.DOI:10.19662/j.cnki.issn1005-2755.2025.03.005.
基金信息:
海关总署“揭榜挂帅”科研项目（2024HK101）; 宁波市公益性科技计划重点项目（2023S059）