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下载次数 | 被引频次 | 阅读次数 |
根据mtDNACOI基因的部分序列,设计出两对西花蓟马特异性引物,建立起西花蓟马的PCR鉴定方法。本方法适用于西花蓟马的成虫、若虫及蛹的快速检疫鉴定。
Abstract:In this study, two pairs of specific primer were designed based on the partial sequence of the COI gene of mtDNA of Frankliniella occidentalis, the PCR detection method was established, which is applicable to rapid quarantine identification of adults, larvae and pupa of the pest.
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4Brunner P C,Fleming C,Frey J E.A molecular identification key for economicallyimportant thrips species(Thysanoptera:Thripidae)using direct sequencing and a PCR-RFLP based approach.Agricultural and Forest Entomology,2002,4:127~136.
基本信息:
DOI:
中图分类号:S433
引用信息:
[1]周力兵,刘忠善,李春艳等.PCR法鉴定西花蓟马[J].植物检疫,2007(02):78-81.
基金信息:
云南省科技攻关项目2005NG01