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烟草环斑病毒是我国检疫性有害生物,为防止该病毒传入国内,需建立快速的诊断和检测方法。将烟草环斑病毒的多克隆抗体吸附到磁性纳米颗粒上,研制出磁免疫层析检测试纸条。该试纸条可特异性检测烟草环斑病毒,检测部分提纯病毒的灵敏度为1μg/mL,检测烟草病汁液可稀释1000倍(重量/体积)。该试纸条和线虫传多面体病毒属的番茄环斑病毒、番茄黑环病毒、南芥菜花叶病毒和葡萄扇叶病毒未出现交叉反应,可用于烟草环斑病毒的现场检测。
Abstract：Tobacco ringspot virus(TRSV)is a quarantine pest for China. In order to prevent the introduction of the virus into China, we need to establish a rapidly diagonstic method for use at the point-of-care or in the fi eld. Magnetic immunochromatography assay was developed by coupling magnetic nanoparticles with polyclonal antibody against TRSV. The sensitivity of the method was 1μg/mL for detecting partial purifi ed TRSV. Extract from TRSV infected Nicotiana tabacum cv. White Burley leaf was detected to a dilution of 10-3(W/V). The immunostrip was specifi c to TRSV. There was no cross reaction with other nepoviruses such as Tomato ringspot virus, Tomato black ring virus, Arabis mosaic virus and Grapevine fanleaf virus. The immunostrip can be used for the on-site detection of TRSV.
[1]姚文国.中国进境植物有害生物选编.北京:中国农业出版社,1997.
[2]魏梅生,李桂芬,张周军,等.胶体金免疫层析法快速检测烟草环斑病毒.植物检疫,2002,16(2):81-83.
[3]封立平,陈长法,孙伟,等.电化学酶联免疫分析法检测烟草花叶病毒和烟草环斑病毒.检验检疫科学,2003,13(2):4-5,3.
[4]相宁,周雪荣,孙彤,等.RT-PCR检测烟草环斑病毒的研究.植物检疫,1995,9(6):337-339.
[5]杨伟东,郑耘,陈枝楠,等.烟草环斑病毒RT-Realtime PCR检测方法.植物保护学报,2007,34(2):157-160.
[6]闻伟刚,崔俊霞,盛蕾.烟草环斑病毒和番茄环斑病毒的半巢式RT-PCR检测.植物保护学报,2007,34(1):61-66.
[7]Wu W,He Q G,Jiang C Z.Magnetic iron oxide nanoparticles:synthesis and surface functionalization strategies.Nanoscale Res Lett,2008,3:397-415.
[8]魏梅生,李桂芬,陈燕芳,等.烟草环斑病毒无侵染性组分的分离.植物检疫,2000,14(4):193-195.
[9]邓丛良,江明,汪万春,等.应用MNP-RT-PCR方法检测黄瓜绿斑驳花叶病毒.植物病理学报,2008,38(4):436-440.
[10]阚春月,衣杰荣,王守法,等.免疫磁珠结合RT-PCR检测黄瓜绿斑驳花叶病毒.上海农业学报,2010,26(4):43-47.
[11]Liu C Y,Jia Q J,Yang C H,et al.Lateral flow immunochromatographic assay for sensitive pesticide detection by using Fe3O4 nanoparticle aggregates as color reagents.Anal Chem,2011,83:6778-6784.
[12]孙健斌,王伟华,雷小英,等.纳米磁性颗粒标记的免疫层析法定量检测人乙型肝炎病毒表面抗原.生物技术通讯,2012,23(1):94-97.
[13]孙健斌,雷小英,包晗,等.纳米磁性颗粒标记的免疫层析法定量检测人绒毛膜促性腺激素.现代生物医学进展,2011,11(24):5001-5004.
基本信息:
DOI：
中图分类号:S435.72
引用信息:
[1]魏梅生,李桂芬,马洁等.烟草环斑病毒磁免疫层析试纸条的研制[J].植物检疫,2014,28(02):24-27.
基金信息:
质检公益性行业科研专项(201310071)